FlowCAP - Flow Cytometry: Critical Assessment of Population Identification Methods

Flow cytometry has been widely used by immunologists and cancer biologists for more than 30 years as a biomedical research tool to distinguish different cell types in mixed populations based on the expression of cellular markers. It has also become a widely used diagnostic tool for clinicians to identify abnormal cell populations associated with disease. In the last decade, advances in instrumentation and reagent technologies have enabled simultaneous single-cell measurement of tens of surface and intracellular markers, as well as tens of signaling molecules, positioning flow cytometry to play an even bigger role in medicine and systems biology. However, the rapid expansion of flow cytometry applications has outpaced the functionality of traditional analysis tools used to interpret flow cytometry data such that scientists are faced with the daunting prospect of manually identifying interesting cell populations in 20 dimensional data from a collection of millions of cells. For these reasons a reliable automated approach to flow cytometric analysis is desirable. While there has been a growing interest among the scientific community in developing these methods, guidance for end users about appropriate use and application of these methods is scarce.

In response to this need, we are pleased to announce the Flow Cytometry: Critical Assessment of Population Identification Methods (FlowCAP) project. The goal of FlowCAP is to advance the development of computational methods for the identification of cell populations of interest in flow cytometry data. FlowCAP will provide the means to objectively test these methods, first by comparison to manual analysis by experts using common datasets, and second by prediction of a clinical/biological outcome.

 

FlowCAP-IV Summit

A summit will be held as part of the CYTO conference from 17 to 21 May 2014. Participation is open to all conference attendees.

FlowCAP-IV Materials

·  Call for Participants: [pdf]

·  Access to data: rbrinkman [at] bccrc [dot] ca

 

FlowCAP-III Summit

An NIH/NIAID-sponsored summit was held at the NIH campus from 29 to 30 Nov 2012 and open to the community through generous sponsorship by ISAC and NIH/NIAID.
For more details, please visit here.

FlowCAP-III Materials

·  Call for Participants: [pdf]

 

FlowCAP-II Summit

An NIH/NIAID-sponsored summit was held at the NIH campus from 22 to 23 Sept 2011. Participation was by invitation only. Participants in the FlowCAP challenges were invited to present their work.
For more details, please visit here.

FlowCAP-II Materials

·  Call for Participants: [html]

·  Call for Datasets: [html]

 

FlowCAP-I Summit

An NIH/NIAID-sponsored summit was held at the NIH campus from 21 to 22 Sept 2010. Participation was by invitation only. Participants in the FlowCAP challenges were invited to present their work.
For more details, please visit here.

FlowCAP-I Materials

·  Mailing List: [html]

·  Time Line: [html]

·  Call for Datasets: [pdf]

·  Participant Guide: [pdf]

·  Dataset Description: [pdf]

·  Data Formatting Submission Example: [pdf]

·  FlowCAP Summit 2010: [html]

 

Organizing Committee

·  Ryan Brinkman, British Columbia Cancer Agency

·  Raphael Gottardo, Fred Hutchinson Cancer Research Center

·  Tim Mosmann, University of Rochester

·  Richard H. Scheuermann, J. Craig Venter Institute

 

Core Developers

·  Nima Aghaeepour, University of British Columbia

·  Greg Finak, Fred Hutchinson Cancer Research Center

 

Acknowledgements

·  National Institute of Health

·  National Institute of Allergy and Infectious Diseases

·  British Columbia Cancer Agency

·  Fred Hutchinson Cancer Research Center

·  University of Texas Southwestern Medical Center

·  TreeStar Inc.

·  University of British Columbia

 

Publications and Availability

Please visit here.

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