FlowCAP-III Summit 2012

Nov 29-30, 2012
NIH Campus, Bethesda, Maryland
Natcher Conference Center
Balcony C
45 Center Drive
Bethesda, Maryland 20892
Attending FlowCAP Summit: Attendance of the workshop will be open to the public. Advance registration is recommended through the registration website listed below. Limited travel support available upon request.

FlowCAP-III Summit 2012 assembled the key stakeholders in field to present and discuss the results from the FlowCAP-III competition, and to discuss how automated methods are being used to address biological questions. The FlowCAP project (http://flowcap.flowsite.org/) was established to provide a mechanism to compare and contrast the utility of these novel computational approaches as applied to a common set of reference datasets.
The objectives of this two-day workshop were to:


Day 1: Nov 29

Session 1 – Informatics Challenges in Flow Cytometry (9:00am-12:00pm)

This session explored some of the current challenges faced by the flow cytometry (FCM) community in analyzing flow cytometry and high dimensional single cell data.

9:00am  Welcome address
9:10am Daniel Rotrosen (NIH)

9:12: Ryan Brinkman, BBCRC FlowCAP2 results update and manuscript status [Slides]
9:15-10:15am Keynote: Carlos Eduardo Pedreira & Quentin Lecrevisse, (Euroflow)
Title: “Automated MRD detection in  B-CLPD: novel strategies developed by the
Euroflow Consortium”

10:15-10:45am Break

10:45-11:15am “A mine is a terrible thing to waste: Extracting the most from polychromatic flow cytometry datasets”, Mario Roederer (NIH)
11:15-11:45am Lyoplate update, Phil McCoy, (NIH)

12:00-1:00pm Lunch on your own

1:00pm:1:30pm “High dimensional single cell assays”, Pratip Chattopadhyay, (NIH)


Session 2 – FlowCAP-III Challenges & Results (1:00pm-5:00pm)

Talks by data providers discussing the datasets, their evaluation criteria and results (FlowCAP-CC). Each challenge and its results was discussed in turn.
Each presentations are 25mins + 5mins for questions.

1:30pm EQAPOL data, Cliburn Chan [Slides]& Nima Aghaeepour [Slides]

2:00pm HVTN data, Steve de Rosa & Greg Finak
2:30pm Lyoplate data, Phil McCoy & Greg Finak
3:00pm General review and discussion of results by FlowCAP committee

3:30pm-4:00pm Break

Session 3 – New Algorithms for FCM Analysis and their application to FlowCAP-III 4:00pm-5:00pm

This session included presentations from those groups who have developed novel methods for FCM data processing and analysis not presented in previous FlowCAP challenges and groups that have developed significant modifications to previous methods that have resulted in substantial improvements.
4:00pm-4:15pm Tim Mosmann, University of Rochester [Slides]
4:15pm-4:30pm John Ramey, Fred Hutchinson Cancer Research Center
4:30pm-4:45pm Bartek Rajwa, Purdue University
4:45pm-5:00pm Till Sörensen, Charité - Universitätsmedizin Berlin


5:00pm Social time & Group dinner.

Everyone welcome. Location to be determined. (Note: Participants are responsible for their meal).


Day 2: Nov 30

Session 4 – New Algorithms for FCM Analysis and their application to FlowCAP-III, Continued (9:00am-10:30am)

Presentations from individual participating groups continued.
Aaron Brandes, Broad Institute [Slides]
Rick Stanton, JCVI
Peng Qiu, MD Anderson [Slides]
Ryan Brinkman, BCCA [Slides]

Ariful Azad, Purdue [Slides]



Session 5 – Future of Flow Informatics 11:00am -  1:00pm

An open session dedicated to the review of the state-of-the-art and planning of FlowCAP. Everyone was welcome to participate!


FlowCAP 4 – plans [Slides]

1:00- Lunch and departure